Gut microbiota community and functions of mouse exposed to single As, single Fe, and combined As and Fe

Mice were fed with pure water, 3mg/L As, 5mg/L Fe, and 3mg/L As + 5mg/L Fe for 90 days. Then, the fecal samples were collected, and genomic DNA was extracted by FastDNA SPIN Kit for Soil (Qiagen, CA). Genomic DNA was amplified with a set of primers targeting the hypervairable V3 and V4 regions of 16S rRNA gene. The PCR products were quantified using Nanodrop and was sent out for pyrosequencing on the Roche 454 FLX Titanium platform. High-throughput sequencing for mouse fecal genomic DNA using Illumina Hiseq 2000 was performed to identify the function genes of gut microbiota.