Data for "Transcriptomic and Functional Responses of Human Airway Cells to Vaped ∆8-THC and its Oxidation Product ∆8-THCQ"

This dataset contains the underlying raw data, chemical analyses, and functional imaging supporting research into the respiratory risks associated with Delta-8-tetrahydrocannabinol (Delta-8-THC) vaping products. The study identifies Delta-8-THC quinone (Delta-8-THCQ) as a major reactive electrophile in commercial distillates that increases significantly upon aerosolization, reaching millimolar concentrations.
The repository includes the following data components:
RNA-Seq Raw Counts: Cell Line (16HBE): Transcriptomic responses to direct exposure of Delta-8-THC (8.5 µM) vs. Delta-8-THCQ (8.5 µM) compared to a vehicle control.Primary Cells (HBECs): Transcriptomic profiles of primary differentiated human bronchial epithelial cells exposed to vaped Delta-8-THC aerosols using the UNC Vaping Product Exposure System (VaPES) and commercial products.Chemical Analysis Data: Quantification of Delta-8-THCQ and other constituents in commercial Delta-8-THC distillates, disposable vapes, and juice products before and after vaping. Chemical analysis was done by LC-MS and GC-MS.Functional Cilia Analysis: Representative videos demonstrating impairment of motile-cilia activity following exposure to distillate and disposable aerosols (5 or 10 puffs)Key Findings:
While Delta-8-THC alone showed minimal transcriptomic impact, its byproduct Delta-8-THCQ induced marked changes in cilia-related, xenobiotic-metabolism, and fibrosis-linked signaling. These data suggest that repeated inhalation of Delta-8-THC products may disrupt mucociliary defense and promote chronic airway injury through the generation of reactive quinones.