Next Generation Sequencing to Identify Targets of the C. albicans Dfi1 Pathway

Purpose: To identify downstream genetics targets of the Dfi1 pathway during Dfi1 activating conditions. Methods: WT and dfi1 null cells were grown in minimal media, then treated with either 4uM of the calcium ionophore A23187 or an equal volume of 100% ethanol vehicle. After 30 min of treatment, cells were collected for RNA extraction and analysis. RNA was extracted using the Qiagen RNAeasy kit, then sent to the Tufts University Core Facility for library prep and sequencing. Results: Analysis of this data resulted in the identification of a number of possible targets of the Dfi1 pathway, as reported in the manuscript "Bypass of Dfi1 regulation of Candida albicans invasive filamentation by iron limitation". Overall design: mRNA profiles of WT and dfi1 null cells treated with the calicium ionophore A23187 or vehicle control, in triplicate.