In situ Hi-C study of chromatin conformation during early Drosophila development. In situ Hi-C study of chromatin conformation during early Drosophila development

In this experiment we examined how chromatin is structured during early Drosophila melanogaster development. We performed in situ Hi-C for hand-sorted non-mitotic embryos at nuclear cycle number 12, 13 and 14, and for embryos at 3-4 hours post fertilisation. During this time in development, the zygotic genome is activated and zygotic transcription is taking place for the first time. To assess the impact of transcription on chromatin structure we use the transcription inhibitor alpha-amanitin.