Mass spectrometry-based identification of proteins from murine and human cell samples analyzed under ATP stimulation conditions

This dataset contains the results of protein identification by nanoLC–ESI–MS/MS after SDS-PAGE separation and in-gel tryptic digestion. Protein bands were manually excised from Coomassie Blue–stained gels and processed for peptide extraction and mass spectrometric analysis. Proteins were extracted from murine macrophage lineage cells (J774.G8), human monocyte lineage cells (U937), and primary mouse cells, analyzed under conditions with and without ATP stimulation. Peptides were analyzed using a Waters NanoAcquity UPLC system coupled to a Q-Tof quadrupole/orthogonal acceleration time-of-flight spectrometer (Waters, Milford, MA). Data acquisition was performed with MassLynx (v4.1), and spectra were processed using the ProteinLynx Global Server (v2.5). Protein identifications were obtained with the Mascot search engine (v2.3; Matrix Science) against the NCBInr protein database. Search parameters included: Enzyme: Trypsin (one missed cleavage allowed) Fixed modification: Carbamidomethyl (C) Variable modification: Oxidation (M) Mass tolerance: 0.1 Da (MS and MS/MS) Positive identification: ≥2 peptides with scores >42 The dataset includes: The processed spectra files (.pkl) The identification results (.xlsx) Representative screenshots of Mascot search results (.png) A metadata and methods summary file (README.txt) These data support the manuscript describing protein identification from murine and human cells analyzed under ATP-stimulated and non-stimulated conditions. Instrument: Waters Q-Tof (NanoAcquity system)Software: MassLynx v4.1; ProteinLynx Global Server v2.5; Mascot v2.3Database: NCBInrData type: Processed MS/MS spectra and identification resultsFile formats: .pkl, .xlsx, .png, .txtContact: Carla Santos de Oliveira - [e-mail]; [e-mail]; [e-mail]: CC-BY 4.0