Digestion of NMDA-R associated proteins following activation of resident calpain-1.
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SK-N-BE cells were exposed to the indicated stimuli for 30 min at 37°C. The deoxycholate-soluble cell fraction was prepared as described in Methods and submitted to immunoprecipitation using anti-NR1 antibody. The immunoprecipitated material was analyzed by immunoblotting for the indicated proteins. The data shown are the quantification of the immunoreactive signals expressed as arbitrary units (means ± SD of five different experiments). Digestion of NMDA-R associated proteins following activation of resident calpain-1.
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2015-01-09
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