miRNA expression profiles of aneurysmal tissues

To identify miRNAs which could be involved in TAA (Thoracic Aortic Aneurysm) pathogenesis, we first performed miRNAs microarrays using the aortic media of healthy controls and TAA patients. 473 miRNAs were detected in at least half samples. Among them, 232 miRNAs were differentially expressed with adjusted p value < 0.05 and log2 transformed fold change > 0.5 or < -0.5. Total RNA was extracted from aortic media of 19 patients and 19 healthy controls and was hybridized with Agilent microarray G3 Human miRNA Microarray Kit, which contained 5893 probes corresponding to 2549 miRNAs from miRBase release 21.