S5A-C Figure

S5 Fig. AP-1 and NF-κB may be more active in IFN-β high cells. BC3-IFN-βp-tdTomato reporter cells were treated with 20 ng/ml TPA for 48 hours to induce the lytic cycle (“lytic (+TPA)”), 10 μM of the pan-caspase inhibitor IDN-6556 (“casp-i”), and a cocktail mix of antibodies against type I IFNs and their receptor (“anti-IFN Abs”) to block IFN-β paracrine signaling. Protein was isolated from BC3-IFN-βp-tdTomato reporter cells without sorting (“bulk”), or after sorting the lytic+casp-i+anti-IFN Abs based on tdTomato expression. Western blots were probed for phosphorylated ATF2, phosphorylated c-Jun, and β-actin as a loading control. Levels of phosphorylated ATF2 and phosphorylated c-Jun in sorted samples were quantified from the western blots, normalized to β-actin, and plotted relative to bulk lytic+casp-i+ anti-IFN Abs samples. ns = p>0.05, * = p