Miguel Vicente-Manzanares, Karen Newell-Litwa, Alexia I. Bachir, Leanna A. Whitmore, Alan Rick Horwitz (2011) CIL:25844, Cricetulus griseus, epithelial cell. CIL. Dataset

Time-lapse microscopy of a spreading MIIB (myosin II B)-deficient cell cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin. Arrows point to clusters, or proto-bundles, denoted by the accumulation of MIIA (the only isoform present in the knockdowns, labeled with the RLC-D,D–GFP) that form and disassemble rapidly. Images were captured in TIRF mode using an inverted microscope (IX70; Olympus) coupled to a CCD camera (Retiga Exi; Qimaging). Live samples were acquired at 37°C using CCM1 medium. This movie corresponds to Fig. 1 D. and Video 2 from J Cell Biol 2011 193:381-396.

本数据集展示了在纤维连接蛋白上表达RLC-D,D–GFP（一种RLC的磷酸模拟突变体）的缺失MIIB（肌球蛋白II B）的细胞，其时序显微观察。箭头所指为聚集或原束，由MIIA（敲低实验中仅有的存在形式，标记为RLC-D,D–GFP）的积累所形成，这些结构快速形成与解体。图像采用倒置显微镜（IX70；Olympus）和耦合的CCD相机（Retiga Exi；Qimaging）在TIRF模式下获取。活细胞样本在37°C下使用CCM1培养基进行采集。本视频对应于J Cell Biol 2011年第193卷第381-396页中的图1D和视频2。