wetland metagenome Metagenome

Representative soils collected from a restored tidal freshwater wetland and a nearby natural “reference” marsh were used in experimental manipulations designed to quantify the effects of soil texture and plant litter quality on C mineralization and microbial community structure. The texture of soils collected from a restored tidal freshwater wetland were manipulated by increasing the clay content from 13% to 20% and 30%. Soil from a nearby natural “reference” wetland containing 42% clay was used as a comparison. Soil microcosms were continuously monitored over a 9-week period to track C mineralization of contrasting plant organic C inputs. Microcosms were destructively sampled at three time points to track changes in microbial community composition and soil physiochemical properties. Libraries of the 16S ribosomal RNA gene were constructed following Illumina’s 16S rRNA metagenomic sequencing library preparation protocol. Pair-end reads were generated on a MiSeq platform using a 600-cycle v3 Reagent Kit.

在本次实验中，选取了自潮汐淡水湿地恢复区域以及邻近的天然“参照”湿地中的代表性土壤样本，用于探究土壤质地与植物凋落物质量对碳矿化及微生物群落结构的影响。通过将潮汐淡水湿地恢复区域采集的土壤样本的粘土含量从13%提升至20%和30%，对土壤质地进行了操控。以含有42%粘土的邻近天然“参照”湿地土壤作为对比样本。在为期9周的实验期间，持续监测土壤微域以追踪不同植物有机碳输入的碳矿化过程。在三个时间节点对微域进行了破坏性采样，以追踪微生物群落组成及土壤物理化学性质的变化。遵循Illumina的16S核糖体RNA基因宏基因组测序文库制备协议，构建了16S核糖体RNA基因文库。在MiSeq平台上使用600循环v3试剂盒生成对端测序读段。