Daniel C. Worth, Kairbaan Hodivala-Dilke, Stephen D. Robinson, Samantha J. King, Penny E. Morton, Frank B. Gertler, Martin J. Humphries, Maddy Parsons (2011) CIL:12286, Mus musculus, fibroblast. CIL. Dataset

This confocal image of wild type cells plated on cell-derived matrices that correspond to an accompanying image of beta3-/- integrin cells. There are ncreased numbers of peripheral matrix-associated adhesions in the beta3-/- cells. The cells are co-stained for active beta1 integrin (9EG7-FITC, green), paxillin (Alexa-568, red) and fibronectin (Alexa-633, blue). Images were acquired using a Zeiss LSM 510 Meta microscope, 1024x1024 resolution, 13 stacks in the Z plane at 0.5um intervals. Images correspond to Fig 1C in J Cell Bio. 2010. 189:369-383

本为野生型细胞在细胞衍生的基质上 plated 的共聚焦图像，与附带的 beta3-/- 整合素细胞图像相对应。beta3-/- 细胞中周围基质相关粘附物的数量有所增加。细胞同时染色以检测活性 beta1 整合素（9EG7-FITC，绿色）、paxillin（Alexa-568，红色）和纤连蛋白（Alexa-633，蓝色）。图像采用蔡司 LSM 510 Meta 显微镜获取，分辨率为 1024x1024，Z 平面内以 0.5 微米间隔拍摄 13 层。图像与《细胞生物学杂志》2010 年第 189 卷第 369-383 页的图 1C 相对应。