Data_Sheet_1_Blood Metagenome in Health and Psoriasis.PDF

A survey and analytical assessment of the results of fundamental works on studying blood metagenome (set of all non-human DNA) is carried out. All works on determining bacterial DNA concentration in the whole blood of healthy people are reviewed. Detailed comparison of characteristics of 16S rRNA test (hereinafter 16S-test) and whole metagenome sequencing test (hereinafter WMS-test) is carried out and published in Supplement S1. One of main goals of this review is to identify the drawbacks and mistakes which the studied works contain, particularly to emphasize the crucial importance of determining total concentration of bacterial DNA for comparing patients' metagenomes with those of healthy people as well as for comparing patients' metagenomes with each other. Controlling the level and composition of contamination is equally important. The absence of high-quality contamination control at each step (or at certain steps) of the research significantly reduces the reliability of achieved results. The given review is the first attempt to analyze and systematize the results of blood metagenome studies, whose number has increased considerably in the last few years. The review has been carried out as part of preparation for implementing a project on complex studying metagenomes of whole blood and skin biopsies of psoriatic patients.

本项研究对研究血液宏基因组（所有非人类DNA的集合）基础工作的成果进行了调查和分析评估。对确定健康人群全血中细菌DNA浓度的所有工作进行综述。对16S rRNA测试（以下简称16S-test）和全宏基因组测序测试（以下简称WMS-test）的特征进行了详细的比较，并将结果发布在补充材料S1中。本综述的主要目标之一是识别被研究工作中存在的缺陷和错误，特别是强调确定细菌DNA总浓度对于将患者宏基因组与健康人群的宏基因组进行比较，以及将患者宏基因组相互比较的至关重要的意义。控制污染物的水平和组成同样至关重要。研究过程中（或某些阶段）缺乏高质量的污染控制显著降低了所得结果的可靠性。本综述是对近年来血液宏基因组研究数量显著增加的首次分析和系统化尝试，也是为了实施一项关于研究银屑病患者全血和皮肤活检复杂宏基因组项目所做的准备工作。