Thiostrepton modifies PRX3, increases mitochondrial H2O2 and disrupts mitochondrial bioenergetics in MM cells.
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https://figshare.com/articles/dataset/_Thiostrepton_modifies_PRX3_increases_mitochondrial_H_2_O_2_and_disrupts_mitochondrial_bioenergetics_in_MM_cells_/1427252
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(A) Recombinant PRX3 (rPRX3) was incubated with indicated concentration of thiostrepton (TS) for 30 min and visualized by immunoblotting with anti-PRX3 antibody after separation by reducing SDS-PAGE. (B) Mitochondrial redox status was analyzed by ratiometric live cell imaging of mito-roGFP in HM cells treated with 5 μM TS for 6 hr (n = 10 cells). (C) Hydrogen peroxide production in isolated mitochondria respiring on succinate (+ succinate) in the presence of indicated compounds (TS = thiostrepton, ROT = rotenone) (* p S2 Fig.
创建时间：
2016-02-23
