Microglia-specific gene transcription mechanism during inflammation response

Microglia are resident immune cells in the central nervous system that regulate the innate and adaptive immune systems. Gene transcription in immune cells is regulated by various toll-like receptor signaling pathways based on the type of stimulus. To identify microglia-specific transcription mechanisms, we performed whole transcriptome shotgun sequencing and chromatin immunoprecipitation followed by high-throughput DNA sequencing for PU.1 binding regions after LPS treatment (10 ng/ml) in microglia.