RNASeq analysis of murine bone marrow derived macrophages (BMDMs)

Macrophages can adapt and change various activation states. Pro-inflammatory macrophages are induced by stimulation with LPS or IFNy whereas repair macrophages are induced by stimulation with IL4/IL13. However, due to their plasticity macrophages might adapt several activation states including inflammatory- and repair-like activation states as well as intermediate activation states which are associated with distinct gene expression patterns. Here we analyse murine bone marrow derived macrophages (BMDMs) which have been untreated, treated with IL4/IL13, or IL4/IL13+TNFa. For each treatment condition we analyzed six replicates for a total of 18 samples by bulk RNASeq.