Male maturation measures by rearing treatment.

Mean pituitary mRNA (follicle stimulating hormone beta-subunit, fshb; luteinizing hormone beta-subunit, lhb), testis mRNA (anti-Mullerian hormone, amh; insulin-like growth factor-3, igf3) (relative mRNA expression); gonadosomatic index (GSI; %); and plasma 11-ketotestosterone (11KT; ng/ml) of juvenile male steelhead sampled at Winthrop National Fish Hatchery in release years 2011–2015 separated according to rearing treatment. Data are mean ± SEM. Sample sizes represent total males analyzed for each measure as noted in parentheses. Due to logistical constraints, we were unable to measure testis amh and igf3 in all years of the study. GSI is not included for release year 2014 and 2015 because mature male testes were not weighed. (XLSX)