Molecular data used to test species boundaries, characterize phylogeographic patterns of genetic diversity, and guide the Endangered Species Act listing decision for a North American freshwater mussel species complex

Here we provide the molecular datasets and metadata associated with specimens used in the investigation of species boundaries and phylogeographic structure of two freshwater mussels, one common (Pleurobema sintoxia) and one being considered for protection under the Endangered Species Act (Pleurobema rubrum).