Identification of the target genes of androgen in skeletal muscle using myofiber specific ARKO female mice. Identification of the target genes of androgen in skeletal muscle using myofiber specific ARKO female mice

Androgens have a strong effect against skeletal muscles to increase muscle mass and strength. However, a molecular mechanism of AR action on muscle strength is not clear. To identify the target genes of AR in skeletal muscle, we generated myofiber specific ARKO using HSA-Cre and AR flox mice (cARKO). Nine-week-old female control and cARKO mice were treated with or without DHT for 4 weeks. After euthenization, gastrocunemius muscle were collected and total RNA were extracted. Overall design: mRNA profiles of gastrocunemius muscle obtained from control and cARKO mice treated with or without DHT for 4 weeks were generated by sequencing, in triplicates, using Illumina Myseq.