Average NMJ areas and fibre diameters upon functional impairment of myosin Va.

TA muscles of adult wildtype mice were co-transfected with cytoplasmic YFP and FLAG, FLAG-MCLT, scrambled silMyoVa or silMyoVa, as indicated. Ten days later, transfected muscles were locally injected with BGT-AF647 and imaged with in vivo confocal microscopy. NMJ areas and diameters of corresponding fibres were determined. Data, mean±s.e.m.