Single-cell RNA sequencing from PiKP-785 cancer cells under 4 treatment conditions: Kras* On, Kras* 3d off, Kras* 5d off, and Kras* On off on. Single-cell RNA sequencing from PiKP-785 cancer cells under 4 treatment conditions: Kras* On, Kras* 3d off, Kras* 5d off, and Kras* On off on

Single-cell RNA sequencing analysis was performed on primary pancreatic ductal adenocarcinoma derrived cell line from PiKP (P48-Cre; R26-rtTa-IRES-EGFP; tetO-LSL-KrasG12D/+; Trp53L/L) murine tumors. In this study we establish that oncogenic Kras is the predominant driver of T cell paucity and myeloid infiltration in the pancreatic tumor microenvironment. Then, we use scRNA seq analysis of cultured PiKP cells with and without Kras* extinction to identify immune related genes involved in driving Kras* mediated immune supression in PDAC. Overall design: PiKP-785 cancer cells were cultured under 4 treatment conditions (Kras* On, Kras* 3d off, Kras* 5d off, and Kras* On off on). Cells were cultured in medium with 1 μg/mL Dox to ensure oncogenic Kras expression (Kras* on). To extinguish oncogenic Kras, Dox was withdrawn from the media (Kras* off).