Global knock out of Sirt3 gene in Alzheimer's transgenic mice with amyloid pathology

We have previously demonstrated that Sirt3 gene deletion, a model for metabolic syndrome, leads to brain mitochondrial dysfunction and neuroinflammation. We also reported that silencing of Sirt3 gene in APP/PS1 mice results in exacerbation of insulin resistance, neuroinflammation and beta amyloid plaque deposition. To further understand how metabolic syndrome and amyloid pathology interact, we performed RNA-seq analysis of the brain samples from wild type, Sirt3-/- , APP/PS1 and APP/PS1/Sirt3-/- mice. Comparative gene expression profiling analysis of RNA-seq data from the brain of C57/BL6 (WT), Sirt3-/- (S3), APP/PS1 (AP) and APP/PS1/Sirt3-/- (AP_S3) mice