Disordered Binding Sites Database

Disordered Binding Sites (DIBS) is a repository of protein complexes that are formed by ordered and disordered proteins. Intrinsically disordered proteins (IDPs) do not have a stable 3D structure in isolation and therefore they defy structure determination by X-ray or NMR. However, many IDPs bind to ordered proteins and as a result of the interaction adopt a stable structure. In accord, complex structures involving binding sites in IDPs are available. DIBS offers a collection of these complexes where exactly one protein chain is disordered and all other proteins are ordered (i.e. are stable in their isolated form that is approved by available monomeric structures).

无序结合位点（Disordered Binding Sites, DIBS）是一个收录由有序蛋白与无序蛋白构成的蛋白质复合物的数据库。内在无序蛋白（Intrinsically disordered proteins, IDPs）在游离状态下不具备稳定的三维结构，因此无法通过X射线晶体衍射或核磁共振（Nuclear Magnetic Resonance, NMR）技术解析其结构。然而，多数内在无序蛋白可与有序蛋白结合，并在相互作用过程中获得稳定的三维结构。正因如此，包含内在无序蛋白结合位点的复合物结构得以被解析并公开。DIBS收录了此类复合物的集合，其中仅存在一条无序的蛋白链，其余所有蛋白链均为有序状态，即这些有序蛋白在游离状态下具备稳定结构，且该结论可通过已公开的单体结构得到验证。

搜集汇总

背景与挑战

背景概述

DIBS数据库是一个专门存储内在无序蛋白质（IDPs）与有序蛋白质形成的复合物的资源库，重点收集IDPs在结合时发生折叠并稳定化的结构信息。它提供了大量此类相互作用的数据，包括功能注释、原子级结构细节、结合强度值以及翻译后修饰情况。作为当前最大的IDPs结合有序蛋白质的集合，DIBS与其姐妹数据库共同涵盖了IDPs介导的蛋白质相互作用谱系。