Transcription profiling of Populus x canescens developing and mature xylem under short-days and drought stress treatment

Populus x canescens were kept in climate chambers for a growing period of 8 weeks. Afterwards, treatment started for five weeks in total. Plant were first treated with different daylength (long-days or short-days) under well-watered conditions. After 2 weeks, half of each daylength treatment was drought stressed while the other half was still well-watered. After further three weeks, all poplars were harvested and samples of developing and mature xylem were taken and directly frozen in liquid nitrogen. These samples were taken for RNA extraction and following RNA-sequencing.