Xanthomonas axonopodis pv. glycines strain 12-2 genome sequencing with PacBio

We sequenced a Thai strain of the soybean pathogen Xanthomonas axonopodis pv. glycines, strain 12-2, to look for potential virulence and avirulence factors coded in its genome. For this, we used PacBio sequencing because the long-read technology allows us to accurately assemble clusters of the highly-repetitive TAL effectors, which short-read sequencing technologies do not effectively capture. The genome sequence was a starting point for analyzing genotypic and phenotypic differences between isolates, and for characterizing mutants with changes in virulence.