Ikaros-binding genomic sequences in mouse thymic iNKT cells and total thymocytes (ChIP-seq)

We have conducted ChIP-seq analysis to investigate Ikaros-regulating genes in mouse thymic iNKT cells. Total thyomcytes were also analyzed as a control. Results demonstrate that Ikaros-binding genomic sequences are different between total thymocytes and thymic iNKT cells. Overall design: Thymic iNKT cells were purified from iNKT-iPS cell-derived mice by CD1d-tetramer using MACS magnetic separators. Total thymocytes from wild-type mice and purified thymic iNKT cells were applied to ChIP-seq analysis using anti-Ikaros antibody. ChIP-seq analysis was done by Active Motif.