Transcriptome analysis of NEAT1-silenced and control human coronary artery smooth muscle cells
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA481662
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资源简介:
To examine the effects of NEAT1 depletion on vascular smooth muscle cell (VSMC) transcriptome, human coronary SMCs (HCASMCs) were transfected with scrambled control or NEAT1 silencing RNA duplex (#1) and were harvested to extract total RNA using TRIzol reagent for RNA-seq after 48 h of transfection. Library was prepared using Ribo-Zero gold rRNA removal kit and paired-end sequencing of 100 bases length was performed on a HiSeq 2500 system (Illumina).
创建时间:
2018-07-18



