Transcriptome analysis of NEAT1-silenced and control human coronary artery smooth muscle cells

To examine the effects of NEAT1 depletion on vascular smooth muscle cell (VSMC) transcriptome, human coronary SMCs (HCASMCs) were transfected with scrambled control or NEAT1 silencing RNA duplex (#1) and were harvested to extract total RNA using TRIzol reagent for RNA-seq after 48 h of transfection. Library was prepared using Ribo-Zero gold rRNA removal kit and paired-end sequencing of 100 bases length was performed on a HiSeq 2500 system (Illumina).