soil bacteria strain:multi | isolate:BF, BO Metagenome. soil metagenome

The diversity of bacterial communities from top soil lowland rainforest and oil palm plantation in Sumatra was studied using pyrosequencing of 16S rRNA gene and common biodiversity indices. Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas. Ecological approach used soil pH, TC (Total Carbon), TN (Total Nitrogen), AP (Available Phosphorous) values measurement and bacterial diversity with Shanon and Simpson indices, and bacterial richness with Chao1-ACE index and OTUs. Bacterial diversity and richness in top soil lowland forest and oil palm plantation soil were not different, as soil pH, TC, and N as substrate factors were not different significantly. The majority of sequences related to Acidobacteria (56.33%), Proteobacteria (27.43%), Actinobacteria (7.11%), and Cyanobacteria (5.55%) were from forest; whereas those related to Acidobacteria (50.11%), Proteobacteria (31.63%), Actinobacteria (7.58%), Chloroflexi (2.60%), and Gemmatimonadetes (2.71%) invented from oil palm plantation. Acidobacteria was the most dominant phyla in both habitats, because soil pH in both areas was acidic (3.77 – 4.80 pHH2O). The genera of alphaproteobacteria dominated in genera phylotype of bacterial 16S rRNA phylogenetic revealed both topsoil forest and oil palm plantation. The most genera in phylogenetic tree is Burkholderia from Betaproteobacteria. The bacterial community shift occured in forest transformation, eventough the oil palm plantation showed more bacterial phyla and genera than the lowland forest.