Hoi1 targets the yeast BLTP2 protein to ER-PM contact sites to regulate lipid homeostasis

Membrane contact sites between organelles are important for maintaining cellular lipid homeostasis. Members of the recently identified family of bridge-like lipid transfer proteins (BLTPs) span opposing membranes at these contact sites to enable the rapid transfer of bulk lipids between organelles. While the VPS13 and ATG2 family members use organelle-specific adaptors for membrane targeting, the mechanisms that regulate other bridge-like transporters remain unknown. Here, we identify the conserved protein Ybl086c, which we name Hoi1 (Hob interactor 1), as an adaptor that targets the yeast BLTP2-like proteins Fmp27/Hob1 and Hob2 to ER-PM contact sites. Two separate Hoi1 domains interface with alpha-helical projections that decorate the central hydrophobic channel on Fmp27, and loss of these interactions disrupts cellular sterol homeostasis. The mutant phenotypes of BLTP2 and HOI1 orthologs indicate these proteins act in a shared pathway in worms and flies. Together, this suggests that H..., , # Hoi1 targets the yeast BLTP2 protein to ER-PM contact sites to regulate lipid homeostasis

Dataset DOI: [10.5061/dryad.wdbrv160w](10.5061/dryad.wdbrv160w)

## Description of the data and file structure

This file contains the raw data used to generate the figures produced by the Bashirullah Lab in the preprint: Dziurdzik SK, Sridhar V, Eng H, Neuman SD, Yan J, Davey M, Taubert S, Bashirullah A, Conibear E. Hoi1 targets the yeast BLTP2 protein to ER-PM contact sites to regulate lipid homeostasis. bioRxiv [Preprint]. 2025 Feb 19:2025.02.11.637747. doi: 10.1101/2025.02.11.637747. PMID: 39990326; PMCID: PMC11844476. This manuscript characterizes the function of a novel adapter protein that regulates localization of the bridge-like lipid transfer protein (BLTP) *BLTP2* to endoplasmic reticulum-plasma membrane (ER-PM) contact sites. This dataset includes quantitative reverse transcription PCR (qRT-PCR) data and body size/pupa volume data.

Each file name contains a reference to the figure w...,