• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. • The buffer used was PEM (also known as BRB80) with the inclusion of 8mM D2O. • Images were taken with an Andor
Fetal or adult rat-brain cytosol and fetal rat-brain microtubules contain a high-affinity, low-capacity pregnenolone-binding protein. The equilibrium dissociation constant is in the 30–50 nM range. Th
Time series stack of LLCPK cells stably expressing GFP-EB1, a microtubule tip binding protein. GFP-EB1 fluorescence is seen in microtubules and centrosomes which appear to act as microtubule nucleatio
Flowering plants contain a large number of cyclin families, each containing multiple members, most of which have not been characterized to date. Here, we analyzed the role of the B1 subclass of mitoti
