Data From : Experimental Ecotoxicology Procedures Interfere with Honey Bee Life History

This data set results from an experiment in which we tested the effects of acetone (0.1% in feed), a solvent commonly used to dissolve pesticides, on bees exposed at different developmental stages (larval and/or adult). We analyzed the effect of acetone on the development cycle of bees at the larval stage, using in vitro larval rearing techniques that enable us to precisely control worker exposure. We then analyzed the effect of acetone on the cycle of bees exposed at different stages of development (larval and/or adult), using RFID technology. We explored the potential effect of in vitro larval rearing, combining acetone exposure and treatment conditions (in vitro larval rearing versus in vivo larval rearing). These analyses were repeated over three sessions in May, June and August, to assess the potential effects of solvents on seasonal dependency. Several experimenters carried out the manipulations to rear the bees in the laboratory. We used the techniques of three donor hives, which supplied bees at both larval and adult stages. One hive was equipped with the RFID system, wo sets of four RFID readers (iID2000, 2k6 HEAD; Microsensys) were placed at the entrance to the hive (with a total of eight readers). The two lines of readers allowed us to distinguish the direction of the activity (i.e., an entrance or an exit; Requier et al., ). In particular, we collected values for LSP, AFE,L-AFE, FI, FT, AOF, and foragerData on parameters determined on larvae during in vitro experiments are available in a csv file entitled "larvae-parameters.csv".METADATA data.frame': 2873 obs. of 11 variables: $ Session: Factor variable ; three factors are available, representing the session during which the response was collected (May; June ; August)$ Exposure: Factor variable ; two factors are available, representing larval exposure modalities (C: not exposed; L: larvae exposed to acetone) $ Hive: Factor variable ; three factors are available, representing the hives of origin of the ilarvae ( R3 ; R4 ; R6) $ ID_Plate: a unique identity for each plate used to rear batches of larvae during the experiment $ ID_larvae: Factor variable ; a unique identity for each larva raised in the experiment$ technician_rearing: Factor variable; three factors are available, representing the identitity of the technicians who grafted the larvae at the start of the experimental sessions (a;b and c)technician_transferring: factorial variable; three factors are available, representing the identity of technicians who transferred larvae after feeding (days 7 or 8) to new rearing plates during experimental sessions (a; b c and d).$ Death: binary variable representing the survival or death of individuals ( 0: dead larva ; 1 : living larva)$ Day_death: Numeric variable; representing the day of death of the individual or the date of the end of mortality monitoring. $ Emergence: binary variable representing the emergence of bees after their development during rearing (0: non-emerged bees; 1: emerged bees) $ Day_emergence: Numerical variable; representing the day of emergence of the individuals from the rearing plates. Data relating to the life cycle of bees within hives thanks to RFID data are available in a csv file entitled "Adultbee-parameters.csv".METADATA data.frame: 1655 obs. of 12 variables:$ ID: Factor variable ; a unique identity for each bee monitored by RFID $ Session: Factor variable ; three factors are available, representing the session during which the response was collected (May; June and August) $ Hive : Factor variable ; three factors are available, representing the hives of origin of larval or adult bees (R3 ; R4 ; R6) $ Rearing: Factor variable ; two factors are available, representing the type of rearing the bees followed at the larval stage (in-vitro: reared in the laboratory; in vivo, reared by the bees in the hives). $ Exposure: Factor variable ; four factors are available, representing bees exposure modalities (C: bees that were never exposed to acetone, L: bees that were exposed to acetone only during the larval phase, called larvae exposed, A: bees that were exposed to acetone only during the adult phase, L-A: bees that were exposed to acetone during the larval and adult phases).$ Realease: time variable: date in days represents the time when the individuals were reintroduced to the colony equipped with the rfid system$ LSP:Numeric variable; recording in days up to the last recorded RFID hit for each bee.$ AFE:Numeric variable;recording inthe number of days from the first recorded RFID hit (the first flight)$ L-AFE: Binary variable, the proportion of bees lost after their first exit from the hive (1: bees have not returned from their first trip; 0: bees have returned from their first trip) $ FT: Numeric variable; is the time span from the AFE to the LSP. $ FI: Numeric variable; is the total number of flights performed by bees$ AOF_duration: Numeric variable; determining the AOF of the bees in days using the aof R package (Requier & Rebaudo, 2020)$ AOF_time: Numeric variable; recording the AOF of the bees in days using the aof R package (Requier & Rebaudo, 2020)$ Forager: Binary variable; binary variable representing whether an AOF has been calculated for each bee (0: bees without an AOF ; 1: bees with an AOF)