Extraction of alpha-tomatine from green tomatoes by subcritical water

Green tomatoes, a by-product of agro-food industry, are rich in the glycoalkaloid alpha-tomatine. This compound presents health benefits including anti-inflammatory and fungicide properties. Subcritical water (SWE) extraction, a green and sustainable process, was used to extract tomatine from fresh or frozen tomatoes. SWE extracted ca. 200 mg tomatine/100 g tomato, an amount higher than conventional methods, at a temperature of 190 ̊C and a residence time of 15 minutes. Green tomatoes SWE extracts were characterized in terms of their composition and antioxidant activity. The phenolic content obtained was approximately 200 mg of gallic acid equivalents/100 g tomatoes and the saponin content was 1000 mg of tomatine equivalents/100 g tomatoes. Total carbohydrates content was different between fresh and frozen tomatoes, 1812 mg of D(+)-glucose equivalents/100 g tomatoes versus 1269 mg/100 g, respectively. In terms of antioxidant activity, a value around 100 mg of Trolox equivalents/100 g of tomatoes was obtained in the DPPH assay whereas a value of 558 mg of Trolox equivalents/100 mg fresh tomatoes versus 452 mg of Trolox equivalents/100 g frozen tomatoes were obtained by CUPRAC assay. SWE extraction proved to be a valuable method to extract glycoalkaloids from green tomatoes. The obtained extracts have potential to be used as ingredients and actives in cosmetic and pharmaceutical industry.