microRNA contained in follicular fluid of middle and large follicle.

Follicular fluid is a decisive factor of oocyte quality. This data is smallRNA-seq of follicular fluid collected from large (>10mm in diameter) follicle of ovaries and middle follicles (3-6m in diameter). The follicular fluid was centrifugated to separate supernatant and cellular pellets followed by filuteration (0.2micro m). Exosome RNA was extracted from the supernatant using a SeraMir Exosome RNA Amplification Kit (System Biosciences). The RNA extracted from the follicular fluid were subjected to smallRNA-seq. Three samples were prepared for each small and large antral follicle fluid. RNA quality and concentration were examined using a Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) and a cDNA library for RNAs from FF was constructed using NEBNext Multiplex Small RNA Library kit (New England Biolab, Ipswich, MA, USA). The average length of all derived libraries was confirmed using an Agilent Bioanalyzer with a High Sensitivity DNA Kit (Agilent Technologies, Palo Alto, CA, USA), and the concentration of each library was adjusted to 10 nM based on the qPCR results (KAPA Biosystems, Boston, MA, USA). The multiplexed sample was sequenced as 75 single-read cycles on a NextSeq 500 system (Illumina). Image analysis, base calling, and quality filtering were performed using RTA version 2.4.11 (Illumina). After sequencing, data were process into Fastq using bcl2fastq-v2.20.0.422.