A Brucella ovis HWE histidine kinase is important for detergent resistance.

Through a system-level analysis of non-essential two-component signal transduction systems (TCS) genes in Brucella ovis, we have discovered that deletion of an HWE histidine kinase, BOV_1602, leads to significant increase in resistance to SDS detergent and other anionic or zwitterionic detergents. To better understand this detergent resistance phenotype, we performed RNA-seq experiment using both WT and ?BOV_1602 cultured on no stress (TASB) or TSAB supplemented with 0.004% SDS. Overall design: This RNA-seq experiment was designed to investigate the transcriptome of WT and ?BOV_1602 mutant under no stress condition and under SDS treatment. Cells were cultured on both TSAB (no stress) and TSAB + 0.004% SDS and collected after incubation. We compared the differential gene expression between ?BOV_1602 and WT grown on TSAB and TSAB supplemented with 0.004% SDS. Each strain cultured on each condition had three replicates