TABLE 2 in Xoconochcothelphusa, a new genus for Ehecatusa chiapensis (Rodríguez & Smalley, 1972), with notes on Spirothelphusa Pretzmann, 1965 (Crustacea: Decapoda: Pseudothelphusidae)
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TABLE 2. Primers for the PCR reaction and its thermal profile. GenePrimersAuthorThermal profileCOIF 5’-GGT CAA CAA ATC ATA AAG AYA TYGMeyer 20035’ at 95°C; 35 cycles of 45” of 94°C, 45” at 50°C and 1’ at 72°C; 10’ at 72°C.G-3’R 5’-TAA ACT TCA GGG TGA CCA AAR AAYCA-3’16SF 5’- ACT TGA TAT ATA ATT AAA GGG CCG-3’Palumbi et al. 19915’ at 95°C; 35 cycles of 45” of 94°C, 45” at 50°C and 1’ at 72°C; 10’ at 72°C.R 5’-CTG GCG CCG CTC TGA ACT CAA ATC-3’H3F 5’-ATG GCT CGT ACC AAG CAG ACV GC-3’Colgan et al. 19985’ at 95°C; 35 cycles of 45” of 94°C, 45” at 48°C and 1’ at 72°C; 10’ at 72°CR 5’-ATA TCC TTR GGC ATR ATR GTG AC-3’
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2024-10-15



