RNA splicing programs define tissue compartments and cell types at single cell resolution

Table 1: Splicing summary. Separate tables with the SpliZ and SpliZVD score for each cell and gene for each dataset. The cell, gene, cell type, SpliZ, and SpliZVD are given by the cell, geneR1A_uniq, ontology, scZ, and svd_z0 columns respectively. A: human individual 1 10x; B: human individual 2 10x; C: human individual 1 Smart-seq2; D: human individual 2 Smart-seq2; E: lemur individual 1 10x; F: lemur individual 2 10x; G: mouse individual 1 10x; H: mouse individual 2 10x.Table 2: Differential alternative splicing per compartment. Separate table with the p value based on the SpliZ and SpliZVD for each gene in each dataset, testing differences between compartments. The gene, SpliZ p value, SpliZVD p value, and largest magnitude median for all compartments for SpliZ and SpliZVD for that gene are given by the geneR1A_uniq, perm_pval_adj_scZ, perm_pval_adj_svd_z0, max_abs_median_scZ, and max_abs_median_svd_z0 columns respectively. A: human individual 1 10x; B: human individual 2 10x; C: human individual 1 Smart-seq2; D: human individual 2 Smart-seq2; E: lemur individual 1 10x; F: lemur individual 2 10x; G: mouse individual 1 10x; H: mouse individual 2 10x.Table 3: Differential alternative splicing per cell type. Separate table with the p value based on the SpliZ and SpliZVD for each gene in each dataset, testing differences between cell types. The gene, SpliZ p value, SpliZVD p value, and largest magnitude median for all compartments for SpliZ and SpliZVD for that gene are given by the geneR1A_uniq, perm_pval_adj_scZ, perm_pval_adj_svd_z0, max_abs_median_scZ, and max_abs_median_svd_z0 columns respectively. A: human individual 1 10x; B: human individual 2 10x; C: human individual 1 Smart-seq2; D: human individual 2 Smart-seq2; E: lemur individual 1 10x; F: lemur individual 2 10x; G: mouse individual 1 10x; H: mouse individual 2 10x.Table 4: Most variable splice sites. The most variable splice sites for genes with significant alternative splicing. There is a separate tab for driver sites in human, mouse, and mouse lemur. Each line reports a driver splice site and contains the genomic location information, whether it is an annotated exon and also an exon with annotated alternative splicing, whether the splice site is in 5’ or 3’ UTR of the gene, and whether the lifted over version of the splice site to the other two organisms is found to be a driver splice site, implying a conservation for the driver splice site. Table 5: Regulated alternative splicing events in spermatogenesis. The list of genes with significantly regulated alternative splicing during sperm development. Each line contains information about the number of cells, spearman's correlation, and its p-value for the human gene and also the same information (based on the mouse and mouse lemur sperm data) for its orthologous genes in mouse and mouse lemur genomes.