Selecting for altered substrate specificity reveals the evolutionary flexibility of ATP-binding cassette transporters, Srikant S et al

This is the accompanying data for the Current Biology article titled "Selecting for altered substrate specificity reveals the evolutionary flexibility of ATP-binding cassette transporters" authored by Sriram Srikant, Rachelle Gaudet and Andrew W Murray (https://doi.org/10.1016/j.cub.2020.02.077). The sequence alignment and homology models presented here support the inferences made in our manuscript. The autocrine data presented here is the raw data supporting our figures.

Data index:
MendeleySourceDataFile1.fasta | Alignment of fungal pheromone ABC exporters focused on Y. lipolytica Ste6.
MendeleySourceDataFile2.pdb | Homology model of YlSte6 made based on inward-open LTC4-bound BtMRP1 (PDB:5UJA).
MendeleySourceDataFile3.pdb | Homology model of YlSte6 made based on outward-open ATP-bound BtMRP1 (PDB:6BHU).
MendeleySourceDataFile4.xlsx | Autocrine data of ScSte6, YlSte6, selected clones and mutational combinations of YlSte6 used to test additive model (Figure 6).
MendeleySourceDataFile5.csv | Autocrine data of all selected clones tested by flow cytometry (Figure S2 and S3).

本数据集为《Current Biology》杂志发表的文章《通过选择改变底物特异性揭示ATP结合 cassette转运蛋白的进化灵活性》的配套资料。该文章由Sriram Srikant、Rachelle Gaudet和Andrew W Murray共同撰写（https://doi.org/10.1016/j.cub.2020.02.077）。此处所展示的序列比对和同源模型支持了我们论文中的推断。所提供的自分泌数据为支撑我们图表的原始数据。

数据索引：
MendeleySourceDataFile1.fasta | 针对Y. lipolytica Ste6的真菌信息素ABC转运蛋白的比对序列。
MendeleySourceDataFile2.pdb | 基于 inward-open LTC4-bound BtMRP1（PDB:5UJA）构建的YlSte6同源模型。
MendeleySourceDataFile3.pdb | 基于 outward-open ATP-bound BtMRP1（PDB:6BHU）构建的YlSte6同源模型。
MendeleySourceDataFile4.xlsx | ScSte6、YlSte6选择克隆及其YlSte6突变组合的自分泌数据，用于测试加性模型（图6）。
MendeleySourceDataFile5.csv | 通过流式细胞术测试的所有选择克隆的自分泌数据（图S2和S3）。