Gene annotation for NCMD assembly

For annotating protein-coding genes, RNA samples were prepared and sequenced from 24 different tissues of the Nanchukmacdon individual which was used for whole genome sequencing. Using a combination of ab initio and homology-based prediction approaches with the RNA sequencing data, a total of 20,588 protein-coding genes with an average length of 47.06 Kbp were annotated in the NCMD assembly. Non-coding genes for diverse types of RNAs, including rRNA, snRNA, and miRNA, were annotated by using the Rfam database and Infernal (v.1.1.3). The tRNAscan-SE (v.2.0.5) and RNAmmer (v.1.2) were used to annotate non-coding genes for tRNA and rRNA, respectively.