Integration of Epigenetic and Transcriptomic Signature reveals Key Transcription Factors involving in Renal Tubular Response to extracellular Stimuli

To understand the transcription regulation of renal tubular epithelial cells under stimuli, here we investigated transcriptome, chromatin accessibility and their dynamics through RNA-seq and ATAC-seq under the three types of treatments. We identified genome-wide functional regions which coordinated transcription regulation in human renal proximal tubule epithelial cells (HK2). Our results provide a cell type-specific landscape of chromatin dynamics under stimuli and discovered an important TF in renal tubular epithelial cells that mediated genomic response to different injury stimuli. HK2 cells treated with DMEM/F12, DMSO, H2O2, TGF-B1 and FG4592 were subjected to ATAC-seq and RNA-seq.