Splicing analysis of TP53 exons 3 and 6

This dataset corresponds to a comprehensive study of exons 3 and 6 of the cancer susceptibility gene TP53. For this purpose, we have used a minigene with exons 2 to 9, where we introduced 31 internal microdeletions, one intron replacement and 134 variants of exons 3 and 6 and intron 6 by site-directed mutagenesis that were assayed in SKBR3 cells. We identified four SRE-rich regions essential for exon recognition. Four control ±1,2 and 130 single nucleotide variants (SNVs) located at the SRE-rich intervals were assayed. We found 13 putative SRE-disrupting variants that impaired recognition of exons 3 (△(E3): 10-17%) or 6 (△(E6): 8-26%).