Targeted Manipulation of Cellular RNA m6A Methylation at the Single-Base Level

We engineered m6A methyltransferase with dCas13-M14 to achieve site-specific editing of m6A. The resultant m6A editors can be programmed with a guide RNA, allowing functional comparison of single site methylation in different mRNA regions. Overall design: Examination of m6A methylation patterns after targeted methylation of YTHDF2-1551, SPEN-3525 and GAPDH-782