VIRMA-mediated m6A modification profilling in human nasopharyngeal carcinoma

Several studies revealed an underlying disordered m6A regulatory network in human nasopharyngeal carcinoma (NPC). However, the roles of m6A regulators and sequential m6A dysregulation in NPC remain largely unknown. In the present study, we investigated the role of VIRMA and its underlying mechanisms in NPC. We performed methylated RNA immunoprecipitation sequencing (MeRIP-seq) to identify the potential downstream targets of VIRMA that might be essential in NPC using HONE-1 cells with or without VIRMA silenced (two biological replicates). Total RNA recovered from HONE-1 cells were used to acquire different modification profiles of mRNAs and lncRNAs using MeRIP-seq. In this way, this study reveal the critical role of VIRMA to control the transcription program of NPC, and provide a potential biomarker and therapeutic target for NPC.