Chromatin In Situ Proximity (ChrISP): Single-cell analysis of chromatin proximities at a high resolution. Homo sapiens

Current techniques analysing chromatin structures are hampered by either poor resolution of individual cells or high resolution in only large cell populations. Here we describe a new method, ChrISP,which reproducibly scores for chromatin fiber proximities in 3D in single cells with a resolution of < 170 A. Moreover, ChrISP visualizes higher order structures of individual chromosomes in relation to structural hallmarks of interphase nuclei beyond the resolution of the light microscope. Overall design: Mapping of "unique" (chromosome territory) and "repeated" (COT1) dna used in ChrISP to asses its composition