Development of lncRNA expression of long-term cigarette smoking extract esophageal cell. Development of lncRNA expression of long-term cigarette smoking extract esophageal cell

To invstigate lncRNA expression in long-term cigarette smoking in immortal esophageal cell SHEE, we have employed the microarray lncRNA expression profile as a platform to identify cigaretted-related oncogenes and cancer surpressors. Overall design: SHEE cells were exposed to cigarette smoking extract (15µg/ml) or DMSO for 48 hours. The exposure lasted 30 passages.Two independent experiments were performed.