Expression data of Jmjd2a and Jmjd2c-regulated genes in plasma cell differentiation

Base on the privious studie from our lab, we found the histone demethylases, Jmjd2a and Jmjd2c are upregulated in stimulated primary B cells, depletion of Jmjd2a and Jmjd2c in stimulated B cells resulted in failed activation of B cells. Owimg to the essential functions of Jmjd2a and Jmjd2c, We here applied loss of function approach, such as siRNA, to study the how Jmjd2a and Jmjd2c regulate plasma cell differentiation. Splenic B220+ B cells from total splenocytes were isolated, and treated with Tfh-derived signals. The cells were transfected with sicontrol, siJmjd2a or siJmjd2c to deplete Jmjd2a or Jmjd2c. Collected the cells and lysed the cells directly by adding TRIZOL at day 0, day 1, and day 3 after stimulation.