Subtomogram averaging of helical filaments in cryo-electron tomography

EM processing data related to Zhang et al., 2024 are deposited here. This paper describes a step-by-step protocol to guide subtomogram averaging of helical filaments imaged by cryo-electron tomography. The workflow includes data processing in Warp, IMOD, Dynamo, RELION, M, as well as intermediate steps in MATLAB and other software. The dataset deposited here encompass IMOD alignment data of 20 tilt series (folder: files_for_imod; .xf, taSolution.log, and mdoc files imported to Warp), coordinate files used at 4 times binning (6.78 Ang/pix) in Dynamo (folder: 4bin), coordinate files used at 2 times binning (3.39 Ang/pix) in TOM/AV3 (folder: 2bin), coordinate files used at unbin (1.69 Ang/pix) before RELION refinement as a preparation step (folder: unbin_prep), and coordinate files as the input to and output from RELION and M refinement at unbin (folder: unbin_relion).