Transcriptomic analysis of TCF-7 cKO CD4 T cells

The transcription factor T Cell Factor-1 encoded by (TCF-7) is critical for T cell development. However, the role of TCF-7 on peripheral CD4 T cells mediated allo-immunity was not known. In this report using a clinically relevant model we presented novel finding of how TCF-7 regulates CD4 T cells functions, including CD4 T cells activation, proliferation, differentiation and impacts effector and central memory formation. We uncovered how TCF-7 regulates chemokine receptor which are essential for CD4 T cells migration to the site of inflammation. Our data uncovered how TCF-7 plays critical role in CD4 T cells survival, apoptosis. Using in vivo allo-immunity models and in-vitro studies we demonstrated how TCF-7 plays central role in CD4 T cells mediated damaged to organs like liver, skin and small intestine. We provided both molecular and biochemical and transcriptomic evidence how TCF-7 functionally regulates CD4 T cells mediated apoptosis and cell death, T cell mediated processes, pro-inflammatory and anti-inflammatory cytokines productions in both basal level and after allo-activation. These findings novel findings represent a stem forward to designing target specific approach for CD4 T cells mediated diseases while understand molecular mechanism the role of CD4 T cells in allo-immunity. Overall design: We analyzed FACS purified CD8 T cells of 3 different TCF-7 cKO and WT mouse labeled this group of samples as pre-transplanted samples. For post-transplanted samples 1 x10^6 CD3 T Cells from TCF-7 cKO and WT mice were initially transplanted into lethally irradiated recipent Balb/c mouse and at day 7 post-transplant H2kb+ donor CD4 and CD8 T cells FACS sorted from the spleen of recipients into the Trizol.