Homo sapiens Tell's lab. Homo sapiens

Apurinic/apyrimidinic endonuclease 1 (APE1) is one of the most important enzyme in the DNA base excision repair (BER) pathway. Besides providing a crucial role in the maintenance of genome stability, APE1 acts as a redox-coactivator by regulating the expression of genes involved in tumor progression. Due to its important role in many physiological and pathological processes, including tumorigenesis, aging, angiogenesis, and oxidative stress signaling, APE1 is an emerging target for combination therapy of cancers through the use of DNA-repair or redox inhibitors. However, its causal involvement in the tumorigenic process and in chemoresistance remains to be elucidated. Recent findings have unraveled the novel role of APE1 in RNA metabolism; although the mechanisms and the functional significance remains largely unknown. To identify novel APE1-bound RNAs, we performed RNA-immunoprecipitation followed by high-throughput sequencing (RIP-Seq) in HeLa cells clones expressing an ectopic FLAG tagged wild type APE1 protein. APE1-bound RNA were purified using an anti-FLAG antibody. Experiments were performed in triplicates and to further reduce potential false positives, a negative control of resin lacking the proper antibody was introduced.