microRNA profiles of immune cells and their exosomes. Homo sapiens

Microarray analysis of exosomal miRNAs vs the miRNAs of their respective donor cells. Overall design: To determine the miRNA repertoires of exosomes secreted by immune cells, we isolated exosomes from cell supernatants of the Raji B cell line, the Jurkat-derived J77 T cell line, and primary dendritic cells (DCs) derived from human monocytes. Exosomes were isolated by a series of microfiltration and ultracentrifugation steps