Monitoring fungal community changes by sequencing ITS amplicons in axolotl limb regeneration

In this study, we investigated the fungal community through the sequencing of ITS amplicons at key stages of limb regeneration (0-1-4-7-30-60 days post-amputation, dpa). Briefly, we conducted a longitudinal study, monitoring amputated axolotls for a duration of 60 days. A total of n=21 samples were systematically collected, encompassing 18 limb tissue samples derived from n=54 adult axolotls and 9 individual aquarium samples with replicates.